2024 Phgwfs7.0

Phgwfs7.0

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August undefined, 2024

WebVIB-UGent Center for Plant Systems Biology. Gateway Vectors. Collection. pKGWFS7. WebJan 4, 2024 · JetPhotos.com is the biggest database of aviation photographs with over 5 million screened photos online!

The Plant Journal 64 The plastidial glucose-6 …

WebFeb 28, 2024 · In cold-primed plants, the pleiotropically stress regulated genes CHS (chalcone synthase; At5g13930) and PAL1 (phenylalanine ammonium lyase; At2g37040) were stronger activated by a second cold... WebMar 24, 2024 · We prepared genomic fragments (1) from 4.2 kbp upstream of the translation start site of HAM1 (AT2G45160), which contains the promoter region, to the TS of miR171a; (2) from the TS to the 3′-UTR end; and (3) from the linearized vector (from pHGWFS7.0) by PCR using primer nos. 7 and 8, 9 and 10, and 11 and 12, respectively (see Supplemental ... assaí indaiatuba

Using green fluorescent protein sheds light on Lupinus ... - Springer

WebEnter your name and contact information. To better ensure the security of donor accounts, we need to prove you are a human. We apologize for the inconvenience. Your donation is … WebJan 5, 2024 · Finally, MtCIR1 recombined with pHGWFS7.0 (Supplemental Fig. S3). After sequence confirmation, the terminal construct p MtCIR1 -GUS was transformed into … WebApr 1, 2007 · Mature anthers were fixed in 4% paraformaldehyde and 0.25% glutaraldehyde under vacuum. Fixed samples were then dehydrated through a graded ethanol series followed by a t-butanol series, ... The binary vector pHGWFS7 was modified for the GUS gene under the control of a 2000 ... assaí atacadista taguatinga

A chloroplast envelope membrane protein containing a putative …

BODYGUARD is required for the biosynthesis of cutin in Arabidopsis …

WebThe destination vector (pHGWFS7.0) used here is efficient for promoter expression analysis (Karimi et al. 2002). Site specific recombination properties of Gateway system allowed recombination of the Adh promoter from the pENTR_Adh to … WebGATEWAY -compatible destination vectors (Karimi et al., 2002) for protein subcellular localization (pH7FWG2,0), pro- moter analysis (pHGWFS7,0), overexpression (pH7WG2D,1) and hairpin RNA interference (pH7GWIWG2(I),0) were purchased from the Functional Genomics Division, VIB-Ghent University (Ghent, Belgium). assaí mussurungaWebNdeI for a single cut fragment, L7:1kb + DNA ladder, L1-L6: Digested samples, L2: Positive sample with true single cut. 1E: Plasmid isolation from pHGWFS7.0_Adh vector. assaí maringa aberto

"WebMay 27, 2014 · Sterols have important functions in membranes and signaling. Plant sterols are synthesized via the isoprenoid pathway by cyclization of 2,3-oxidosqualene to … " - Phgwfs7.0

Phgwfs7.0

Subsequent analysis running the decidetests function - Course Hero

WebJan 5, 2024 · Finally, MtCIR1 recombined with pHGWFS7.0 (Supplemental Fig. S3). After sequence confirmation, the terminal construct p MtCIR1 -GUS was transformed into Arabidopsis using Agrobacterium tumefaciens (strain EHA105)-mediated floral dip method (Clough and Bent 1998 ). WebOct 1, 2014 · A sequence of approximately 2kb preceding the translation initiation site of the OsAPx1 gene was isolated, cloned into pENTR vector and recombined in pHGWFS7 vector, whichallows the fusion of the promoter sequence with two report genes, Gfp and Gus, and confers resistence to hygromycin.The construction was named pPROM1. The …

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WebFeb 28, 2024 · Arabidopsis thaliana var. Col-0 wildtype plants and transgenic lines were grown on ... Carlsbad, U.S.A.) into the Gateway site of the vector pHGWFS7.0 101 … WebApr 2, 2010 · Welcome to the Rausher Lab protocol library. Entries which are Wiki pages appear as links; entries which are downloadable files appear with green arrows after their names.

WebOct 20, 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin resistance gene ( HygR) for plant transformation and spectinomycin/streptomycin resistance (Sm/SpR) for bacterial transformation (Karami et al. 2009 ). Web... pHGWFS7 for promoter-gus constructs ( Fig. 1) : This vector with binary pPZP200 backbone ( Hajdukiewicz et al. 1994) contains CmR-ccdB selection flanked by attR1 and …

WebSep 14, 2011 · GATEWAY ™-compatible destination vectors (Karimi et al., 2002) for protein subcellular localization (pH7FWG2,0), promoter analysis (pHGWFS7,0), overexpression (pH7WG2D,1) and hairpin RNA interference (pH7GWIWG2(I),0) were purchased from the Functional Genomics Division, VIB-Ghent University (Ghent, Belgium). The LR reaction was … WebCCATTAACAAATCTCCG-30; and a T-DNA insertion primer, LBb1.3, 5 0-ATTTTGCCGATTTCGGAAC-3 ). The zfp5 gis3, ... RNAi construct, whereas pHGWFS7 was used for the pGIS3:GUS construct. For all cloning constructs, targeted gene-speciﬁc fragments were ﬁrst PCR-ampliﬁed from cDNA

WebFeb 1, 2016 · genomic DNA and the binary vector pHGWFS7.0_RD29A (positive control) were completely digested with BamH1 and the GUS gene was used as probe. Transgenic lines (P-1 and P-2) s howed one band.

Websubsequent analysis Running the decideTests function using the global method from COMM 2024 at Simon Fraser University assaí taubateWebNWCG assaí parauapebasWebThe destination vector is pHGWFS7,0 (13 kb). The donor vector is pDONR221. I attached reference protocols for single-step BP/LR gateway. Any comment and advice will be really … assaí parangaba telefoneWebwith the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants … assaí teresina iningaWebantiporter genes into the GATEWAYTM promoter cloning vector pHGWFS7. The Pokkali promoter expressed the β-glucuronidase or GUS gene ~25-fold more efficiently than the CaMV 35S promoter in rice calli, while that of IR64 was 7-fold ... using PCR mix containing PCR reaction buffer (Invitrogen), 1.33 mM MgCl2, 0.1 mM dNTP mix, 2.66% DMSO, 0.5U … assc casalpusterlengoWebLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors assbah khan assb kuantan