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Phic31 integrase system

WebThe phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid DNA into … WebMay 7, 2013 · We showed in our previous paper that phiC31 integrase works very efficiently in silkworm embryos using an extra chromosomal cassette exchange reaction system; recombination between the attP and attB sites of the plasmid occurred with a high frequency in the silkworm embryos when phiC31-integrase mRNA was supplied by injection and no …

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WebMar 17, 2006 · The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also … WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB … qfm 96 live radio columbus ohio https://holistichealersgroup.com

The phiC31 integrase system for gene therapy - PubMed

WebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non … WebJun 17, 2010 · In addition to the widely used Cre and Flp recombinases, the phiC31 integrase system from Streptomyces phage may also be used for these genetic manipulations in eukaryotic cells. Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … qfm headstone

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Category:phiC31 integrase for recombination-mediated single-copy …

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Phic31 integrase system

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WebAug 1, 2014 · The phiC31 integrase system for gene therapy. Curr Gene Ther 6,633–645 [Google Scholar] Chalberg T.W., Genise H.L., Vollrath D., and Calos M.P. (2005). phiC31 integrase confers genomic integration and long-term transgene expression in rat retina. Invest Ophthalmol Vis Sci 46,2140–2146 [Google Scholar]

Phic31 integrase system

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WebSimply co-transfect with the PhiC31 Integrase Expression Plasmid (Cat.# FC200PA-1) and select for integrants using puromycin resistance and imaging for RFP fluorescence. The bidirectional promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter. WebIn this example, we designed a synthetic system to visualize the long-range interaction between the even-skipped (eve) ... We used BDSC #34770 as the maternal phiC31 integrase source. These female flies provide integrase activity in their germlines, which induces attB/attP recombination to incorporate transgenes. ...

WebJan 1, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in... WebWe developed integrase-mediated site-specific transgenesis in mice via pronuclear microinjection. We use PhiC31 integrase to catalyze recombination between one or two …

The mode integration/resolution and inversion (INT/RES and INV) depend on the orientation of recombinase target sites (RTS), among these pairs of attP and attB. Section C indicates, in a streamlined fashion, the way recombinase-mediated cassette exchange (RMCE) can be reached by synchronous double-reciprocal crossovers (rather than integration, followed by resolution). WebThe system is based on that integrase (PhiC31) catalyzes irreversible recombination between attB and attP sites. We developed integrase-mediated site-specific transgenesis in mice via pronuclear microinjection.

WebMay 22, 2024 · Mammalian system plasmids. For the integrase expression vectors, Ints 2, 4, 5, 7, 9, 13, phiC31, and Bxb1 were placed under the control of the ubiquitin promoter and β-globin poly(A) signal ...

Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 qfm heartbeatWebINTRODUCTION: The phiC31 integrase system is a phage-derived system that offers the ability to integrate plasmid DNA into the chromosomes at a subset of endogenous … qfn share priceWebNational Center for Biotechnology Information qfn and nqfnWebOct 17, 2005 · The phiBT1 phage integrase is an enzyme that catalyses the efficient recombination between unique sequences in the phage and bacterial genomes, leading to the site-specific integration of the former into the latter in a unidirectional manner. qfn crackWebJul 29, 2012 · The phiC31 integrase system. is thought to be more efficient than the FLP recombinase for. the construction of transgenic insects because it has been. successfully applied to diverse organisms ... qfn stand forWebJump-In™ Fast Gateway® System allows you to generate stable clones in just two weeks by introducing your genetic material into your cell background using PhiC31 integrase to … qfmed.comWebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ]. qfn stand off