Phic31 integrase vector system
WebWe developed integrase-mediated site-specific transgenesis in mice via pronuclear microinjection. We use PhiC31 integrase to catalyze recombination between one or two … WebThe PhiC31 Integrase System Popularly used for gene therapy development and related applications, one of the major advantages of SBI’s PhiC31 system is the tight control over …
Phic31 integrase vector system
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WebA polymerase chain reaction assay is also presented to verify correct chromosomal placement of the introduced plasmid. This integration system based on phiC31 integrase supplies a simple method to obtain repeated integration at the same chromosomal site in mammalian cells. Publication types Research Support, N.I.H., Extramural MeSH terms WebMay 12, 2014 · Vector constructs used in this study. (A and B) The genetic structure of the barnase expression vector (“pro-vector”) (A) and the phiC31-integrase-expression vector (B). The provector harbors attP and attB sequences that serve as targets for the site-specific recombinase phiC31. Note that only the T-DNA part of the vectors is illustrated ...
WebJun 1, 2024 · Co-transfection of dual ORF vector with PhiC31 integrase expression vector resulted in FC31 2P cell pools. As the negative control, cells transfected just with single-ORF/IRES or dual-ORF vectors, without phiC31 integrase expression vector. A total amount of 3 μg plasmid DNA was used for each well of the 6-well plate. WebSep 14, 2012 · Abbreviations: phiC31, phage phiC31 recombinase coding sequence [41]; Pubi, maize ubiquitin 1 promoter; Pspm, promoter of the maize suppressor-mutator transposable element spm; intron, sequence derived from an intron of the Petunia hybrida Psk7 gene (GenBank accession number AJ224165); NLS, SV40 T antigen nuclear …
WebOur results demonstrated that dual ORF vector in the presence of phiC31 integrase expression vectors (+FC31 2P) generated more recombinant antibody in comparison to …
WebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non … Lentiviral Expression Plasmids & Lentiviral Vectors - PhiC31 Integrase Expression … Contact Us - PhiC31 Integrase Expression Plasmid System Biosciences Exosome Isolation Kits & Products - PhiC31 Integrase Expression Plasmid System … NTA-based Exosome Characterization - PhiC31 Integrase Expression Plasmid … Exosome Quantitation Kits & Products - PhiC31 Integrase Expression Plasmid … CLOuD9 Gene Expression Regulation System; Targeted Protein Degradation; … Lentiviral Packaging Kits - PhiC31 Integrase Expression Plasmid System Biosciences How it works. Ordering RNA Services is easy. Step 1: Contact the services team … Exosome Biomarker Discovery Products - PhiC31 Integrase Expression Plasmid … Get everything you need for studying and using exosomes, from isolation and …
WebJun 23, 2014 · One possibility is to use the integrase of phage phiC31 (phiC31-int) which catalyzes efficient site-specific recombination between the attP site in the phage genome and the chromosomal attB site of its Streptomyces host. ... Even though phiC31-int has already been delivered by an adenoviral vector system , the use of such vectors is limited … country song my hometownWebpcDNA3.1 phiC31 was a gift from Konrad Basler (Addgene plasmid # 68310 ; http://n2t.net/addgene:68310 ; RRID:Addgene_68310) For your References section: An … brewery middlesbroughWebINTRODUCTION. The Streptomyces phage phiC31 uses a large serine recombinase, phiC31 integrase (Int), to merge its genome with that of its bacterial host [1, 2].Its two substrates … country song my give a damn is brokenWebphiC31 Integrase Vector System pFC-PGK-MCS-EF1-GFP-Puro Dual Promoter phiC31 Donor Vector (Cat# FC551A-1). The cells were split 1:10 after 24 hours post-transfection. … brewery millWebJun 12, 2024 · This study demonstrates that phiC31 integrase protein, whether in the 605- or 613-amino-acid form, is competent to mediate genomic integration when co-nucleofected with a donor plasmid. Both forms of the protein mediated sequence-specific integration, with a tendency for the 613-amino-acid form to be more efficient. country song my churchWebSep 30, 2014 · The phiC31 integrase-based vector system consists of two vector types. One is a donor vector that contains the attB sequence, and the other is a helper vector that expresses phiC31 integrase. PhiC31 integrase expressed by helper vectors integrates donor vectors into chromosomes. brewery milford miWebOver the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells. country song my last single saturday night